Characterization of Pediococcus acidilactici strains isolated from rainbow trout Oncorhynchus mykiss feed and larvae: safety, DNA fingerprinting, and bacteriocinogenicity. The use of lactic acid bacteria LAB as probiotics constitutes an alternative or complementary strategy to chemotherapy and vaccination for disease control in aquaculture. Concerning the safety assessment, none of the pediococci showed antibiotic resistance nor produced hemolysin or gelatinase, degraded gastric mucin, or deconjugated bile salts. The bacteriocin produced by P.
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Pediococcus pentosaceus SL4 was isolated from a Korean fermented vegetable product, kimchi. We report here the whole-genome sequence WGS of P.
The genome consists of a 1. A probiotic bacterium, Pediococcus pentosaceus OZF, isolated from Pediococcus pentosaceus OZF, originally isolated from healthy human breast milk, produces antimicrobial activities against many gram-positive bacterial species, including the food borne pathogen, Listeria monocytogenes. A bacteriocin was purified to homogeneity from the supernatant of exponentially growing cells using Antimicrobial Effect of Bacteriocin produced Pediococcus pentosaceus on some clinical isolates.
Directory of Open Access Journals Sweden. In this study, we investigate, the effect of crude Bacteriocin from Pediococcus pentosaceus on 30 clinical isolates 5 E. In an in vivo experiment, 7-day-old SPF chickens were randomly assigned into three groups. Group 1 served as a control that was fed a basal diet without probiotic supplementation, and groups 2 and 3 were fed the basal diets supplemented with L.
We also determined meat color, fat content, shear force, water content and pH value of breast and thigh muscles; ammonia, urea nitrogen and uric acid content in plasma; fecal ammonia emission level and pH value; and Lactobacillus and Escherichia coli in ceca. Compared with the control group, L. The P. Thus, the additive containing this strain is not considered safe for the target animals and consume Diet supplementation of Pediococcus pentosaceus in cobia Rachycentron canadum enhances growth rate, respiratory burst and resistance against photobacteriosis.
Cobia Rachycentron canadum is an economically important fish species for aquaculture in tropical and sub-tropical areas. Cobia aquaculture industry has severely damaged due to photobacteriosis caused by Photobacterium damselae subsp.
Antibiotics and vaccines have been applied to control Pdp infection, but the efficacy has been inconsistent. The acidic pH derived from metabolic acids in LAB culture supernatant was demonstrated to be an important factor for the suppression. In addition, the respiratory burst RB of peripheral blood leukocytes PBL in the LAB fed group was significantly higher than that of the non-fed group. Judging by the quick induction of high protection against Pdp infection and promotion of growth in larvae, LAB was considered to be a viable probiotic for cobia aquaculture.
All rights reserved. Effect of ethanol and low pH on citrulline and ornithine excretion and arc gene expression by strains of Lactobacillus brevis and Pediococcus pentosaceus. The accumulation of citrulline and ornithine in wine or beer as a result of the arginine catabolism of some lactic acid bacteria LAB species increases the risk of ethyl carbamate and putrescine formation, respectively. Several LAB species, which are found as spoilage bacteria in alcoholic beverages, have been reported to be arginine degrading.
This study evaluates the effect of ethanol content and low pH on the excretion of citrulline and ornithine by two strains belonging to the potential contaminant species Lactobacillus brevis and Pediococcus pentosaceus.
In the conditions that most affected cell viability, arginine consumption per cell increased noticeably, indicating that arginine utilization may be a stress responsive mechanism. In the presence of ethanol, a higher expression of the arcC gene was found in P. This suggests that L. Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 improves acute liver injury induced by D-galactosamine in rats.
This work investigated the effect of the intragastric administration of five lactic acid bacteria from healthy people on acute liver failure in rats. Acute liver injury was induced on the eighth day by intraperitoneal injection of 1. After 24 h, samples were collected to determine the level of liver enzymes, liver function, histology of the terminal ileum and liver, serum levels of inflammatory cytokines, bacterial translocation, and composition of the gut microbiome.
The results indicated that pretreatment with L. Pretreatment with L. To the best of our knowledge, the effects of the three species-L. The excellent characteristics of L. Full Text Available In this study, the infl uence of lactic acid fermentation on the quality of tomato powder was evaluated. The eff ect of adding fermented tomato powder to ready-to-cook minced pork meat to improve its nutritional value and sensory characteristics was also analysed.
The cell growth of Lactobacillus sakei 7. The highest loss up to Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats. Alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy.
Only administration of LI01 or LI05 prevented liver fibrosis and down-regulated the hepatic expression of profibrogenic genes. Metagenomic sequencing of 16S rRNA gene showed an increase in potential beneficial bacteria, such as Elusimicrobium and Prevotella, and a decrease in pathogenic bacteria, such as Escherichia.
These alterations in gut microbiome were correlated with profibrogenic genes, gut barrier markers and inflammatory cytokines. In conclusion, L. These results suggest novel strategies for the prevention of liver cirrhosis.
Enzymatic conversion of D-galactose to D-tagatose: cloning, overexpression and characterization of L-arabinose isomerase from Pediococcus pentosaceus PC The gene encoding L-arabinose isomerase from food-grade strain Pediococcus pentosaceus PC-5 was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. Furthermore, this enzyme exhibited a weak requirement for metallic ions for its maximal activity evaluated at 0.
Interestingly, this enzyme was distinguished from other L-AIs, it could not use L-arabinose as its substrate. In addition, a three-dimensional structure of L-AI was built by homology modeling and L-arabinose and D-galactose were docked into the active site pocket of PPAI model to explain the interaction between L-AI and its substrate.
The purified P. Published by Elsevier GmbH. By using the central composite design CCD we showed that addition of 0. This was higher than any current popular commercial Nham product by roughly 8 times. Based on proximate analysis among the Nham sets, 'GABA Nham' was low in fat, carbohydrate and energy although its texture and color were slightly different from the control TNN. However, sensory evaluations of 'GABA Nham' were more acceptable than the controls and commercial Nham products for all tested parameters.
Supplementation with Lactobacillus paracasei or Pediococcus pentosaceus does not prevent diarrhoea in neonatal pigs infected with Escherichia coli F Infectious diarrhoea is a worldwide problem in newborns.
Optimal bacterial colonisation may enhance gut maturation and protect against pathogenic bacteria after birth. We hypothesised that lactic acid bacteria LAB administration prevents pathogen-induced diarrhoea in formula-fed newborns. In vulnerable newborns there may be a delicate balance among bacterial composition and load, diet and the host. Caution may be required when administering LAB to compromised newborns suffering from enteric infections Bacterial density in colon mucosa increased after F18 inoculation PE Optimization and scale-up of fermentation of glucansucrase and branched glucan by Pediococcus pentosaceus CRAG3 using Taguchi methodology in bioreactor.
Full Text Available The present investigation focuses on screening and optimization of media components to enhance glucansucrase and glucan production by Pediococcus pentosaceus CRAG3 at shake-flask and bioreactor level using Taguchi orthogonal array design. A three-level Taguchi orthogonal array layout of L27 33 was employed, in which six variables were studied for their influence on glucansucrase and glucan production. The results showed that sucrose, K2HPO4 and Tween were the most significant factors to improve glucansucrase production while the glucan production was mostly affected by sucrose, peptone and K2HPO4.
The optimized medium composition for maximum glucansucrase and glucan production were: sucrose 3. The optimized medium gave The optimized medium gave 2 fold enhancement in enzyme activity and 4 fold increase in glucan concentration as compared to non-optimized medium 4.
The obesity and fatty liver are reduced by plant-derived Pediococcus pentosaceus LP28 in high fat diet-induced obese mice. Full Text Available We evaluated the effect of an oral administration of a plant-derived lactic acid bacterium, Pediococcus pentosaceus LP28 LP28, on metabolic syndrome by using high fat diet-induced obese mice.
The obese mice were divided into 2 groups and fed either a high fat or regular diet for 8 weeks. The lean control mice were fed a regular diet without inducing obesity prior to the experiment. No interaction between screen size and microbial phytase on phytate degradation was observed, but the interaction between microbial phytase and incubation time was significant P Evaluation of phytate-degrading Lactobacillus culture administration to broiler chickens. Probiotics have been demonstrated to promote growth, stimulate immune responses, and improve food safety of poultry.
While widely used, their effectiveness is mixed, and the mechanisms through which they contribute to poultry production are not well understood. Microbial phytases are increasingly supplemented in feed to improve digestibility and reduce antinutritive effects of phytate. The microbial origin of these exogenous enzymes suggests a potentially important mechanism of probiotic functionality.
We investigated phytate degradation as a novel probiotic mechanism using recombinant Lactobacillus cultures expressing Bacillus subtilis phytase.
The resulting plasmid, pTD, was transformed into Lactobacillus acidophilus, Lactobacillus gallinarum, and Lactobacillus gasseri. Expression of B. Phytase-expressing L. This will inform future studies investigating whether probiotic cultures are able to provide both the performance benefits of feed enzymes and the animal health and food safety benefits traditionally associated with probiotics.
Phytate degradation by fungi and bacteria that inhabit sawdust and coffee residue composts. Phytate is the primary source of organic phosphorus, but it cannot be directly utilized by plants and is strongly adsorbed by the soil, reducing bioavailability.
Composting is a process used to improve the bioavailability of phytate in organic wastes through degradation by microorganisms. In this study, we aimed to investigate the phytate-degrading ability of fungi and bacteria that inhabit sawdust compost and coffee residue compost, and their contribution to the composting process.
In the plate assay, the fungi that formed clear zones around their colonies belonged to the genera Mucor, Penicillium, Galactomyces, Coniochaeta, Aspergillus, and Fusarium, while the bacteria belonged to the genera Pseudomonas, Enterobacter, Chitinophaga, and Rahnella. Eight fungal isolates genera Mucor, Penicillium, Galactomyces, and Coniochaeta and four bacterial isolates genera Pseudomonas, Enterobacter, and Rahnella were selected to evaluate phytase activity in their liquid culture and their ability to degrade phytate in organic materials composed of mushroom media residue and rice bran.
The selected fungi degraded phytate in organic materials to varying degrees. Penicillium isolates showed the highest degradation ability and Coniochaeta isolate exhibited relatively high degradation ability. The clear zone diameters of these fungal isolates displayed significantly positive and negative correlations with inorganic and phytate phosphorus contents in the organic materials after incubation, respectively; however, none of the selected bacteria reduced phytate phosphorus in organic materials.
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Download full text Bahasa Indonesia, 10 pages. Wicaksana, Bima R. Trash fish were less widely used although they were quite highly potential. The commonly traditional processing smoking, salted boiled fish and fermentation. Fish processing by fermentation for example was fish sauce. One of the weaknesses of the fermentation process was the length of fermentation period.